Table 2.

Primers and PCR conditions used for polymorphisms amplification

Polymorphism	Forward primer	Reverse primer	Annealing temperature
CA-SSR1	5′-ACAAGCTTTTAAAGAGTTTCTTGT-3′	5′-AGGCAATGTGTTAGTACACA-3′	59°C, 35 cycles
-216G/T	5′-GCTTGGTCCTCTTCGGCATCT-3′	5′-CCGTCTTGACCAGTCGCTTA-3′	68°C–60°C decreasing 1°C/cycle for eight cycles, 59°C, 30 cycles
R497K	5′-TTAGATGCAGCATTATTAGCC-3′	5′-CGGGTGATACATCATTAGAAA-3′	59°C, 35 cycles

Abbreviations: CA-SSR1, CA simple sequence repeat in intron 1; PCR, polymerase chain reaction.