Figure 6. Co-localization of PDZD7 with MYO10-MYO7A fusion.
COS7 cells were transfected with the indicated constructs and stained with phalloidin to detect actin. MYO10HMM and MYO10HMM-MYO7Atail constructs (derived from bovine MYO10 and human MYO7A) were fused to GFP, which was imaged directly, as was mCherry fused to the indicated molecules. (A) mCherry-USH1G robustly localizes to filopodia tips when expressed with MYO10HMM-MYO7Atail. (B) mCherry-USH1C generates actin cables, to which MYO10HMM-MYO7Atail targets. (C) mCherry-PDZD7 does not concentrate at filopodia tips when the control MYO10HMM construct is expressed. Occasional exceptions are seen (yellow asterisk). (D) mCherry-PDZD7 does concentrate at filopodia tips when the MYO10HMM-MYO7Atail construct is expressed. (E) Very little Strep-ANKRD24 co-localizes with MYO10HMM-MYO7Atail at filopodia tips. Main panels are 40 × 40 µm; inset panels are 4 × 4 µm.