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Comparison of stereocilia purification methods. The D10 preparation enriches stereocilia membranes, and therefore is not directly comparable to the twist-off method, which isolates the entire hair bundle.
| Parameter | Twist-off | D10 |
|---|---|---|
| Throughput | <40 ears/person/day | >200 ears/person/day |
| Stereocilia purity | >90% | - |
| Recovery | ~1/3 | >1/2 |
| Organs sampled | 1 | 7 |
| Stereocilia protein recovered | 1x | 10x |
| Stereocilia protein per day | 1x | 50x |
| Subsequent purification | Challenging | Easy |
