Table 2.

Rank of predicted binding affinity of the target designed proteins using Patch-Surfer.

Rank	Target protein	Patch-Surfer Score a)	Binding or not
1	DIG5	0.592	Yes (205μM)
2	DIG8	0.653	No
3	DIG19	0.684	Yes (541 pM)
4	DIG10	0.686	Yes (8.9 μM)
5	DIG18	0.688	Yes b)
6	DIG9	0.702	No
7	DIG2	0.711	No
8	DIG3	0.723	No
9	DIG13	0.724	No
10	DIG6	0.729	No
11	DIG7	0.752	No
12	DIG4	0.785	No
13	DIG12	0.789	No
14	DIG17	0.802	No
15	DIG14	0.847	No
16	DIG1	0.963	No

Eight targets in bold (DIG5 to DIG3 in the table) were predicted to bind the target ligand.

^a)A small Patch-Surfer score indicates that the putative binding pocket is similar to the reference steroid binding pocket.

^b)The binding affinity of this protein is not available because it was not reported in the paper by Tinberg et al. ²⁹.