FIGURE 3.

a) Effect of a leukotriene B₄ receptor antagonist on eosinophil trans-basement membrane migration (TBM). Eosinophils (1×10⁵ cells) were treated with BIIL260 (10 μM) and then placed into the upper compartment of a chamber with a Matrigel-coated Transwell insert. Neutrophils (2×10⁴ cells) and LPS (10 µg·mL⁻¹) were placed into the lower compartment. After 120 min of incubation, migrated eosinophils in the lower chamber were measured (n=4). b) Effect of a platelet-activating factor receptor antagonist on eosinophil TBM. Eosinophils (1×10⁵ cells) were treated with WEB2170 (10 μM) and placed into the upper compartment. Neutrophils (2×10⁴ cells) and LPS (10 µg·mL⁻¹) were placed into the lower compartment. After 120 min of incubation, migrated eosinophils were measured by eosinophil peroxidase assays (n=4). Data are presented as mean±sem. Spon: spontaneous migration. *: p<0.05 versus without BIIL260; **: p<0.01 versus without WEB2170.