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. 2016 Jun 28;2(2):00045-2015. doi: 10.1183/23120541.00045-2015

FIGURE 5.

FIGURE 5

Paracrine effects of mesenchymal stromal cells (MSCs) on H292 airway epithelial cells. NCI-H292 airway epithelial cells were incubated with conditioned medium obtained from MSCs (MSC-CM). mRNA expression of epidermal growth factor receptor ligands after 9 h incubation with conditioned medium from MSCs cultured in serum-free low-glucose Dulbecco's modified Eagle medium (LG-DMEM) (Ctrl, from MSC-CMCTRL) or in serum-free LG-DMEM supplemented with TNF-α and IL-1β (Stim, from MSC-CMSTIM). AREG: amphiregulin; HBEGF: heparin-binding epidermal growth factor-like growth factor; TGFA: transforming growth factor-α; CCDN1: cyclin D1. Normalised values compared with housekeeping genes are shown. Individual data points are shown (circles: chronic obstructive pulmonary disease (COPD) donor-derived MSC-CM; squares: non-COPD donor-derived MSC-CM); horizontal bars represent mean. *: p<0.05, n=9 per group. For all genes investigated, mRNA expression was lower in cells cultured in plain culture medium (LG-DMEM without additional stimulation) compared with mRNA expression in cells cultured in MSC-CM (data not shown).