TABLE 3.

Non-synonymous sequence variants of antibiotic resistance genes in Staphylococcus aureus and Pseudomonas aeruginosa populations of cystic fibrosis sputum metagenomes^#

Organism	Gene	Amino acid substitution	Patients n (samples n)
S. aureus	gyrA	p.Ser90Leu	2 (4 and 2 from each patient)
		p.Leu576Phe	1 (1)
		p.Arg843His	1 (1)
		p.Thr833fs	1 (1)
		p.Asp891_Glu892dup	1 (2)
	rpoB	p.Asp320Asn	1 (1)
	parE	p.Asn139Ser	2 (2 and 1 from each patient)
		p.Gly530Asp	1 (1)
P. aeruginosa	mexS	p.Ala235Thr	1 (3)
		p.Arg48His	1 (1)
	mexI	p.Ala782Glu	2 (1 from each patient)

^#: 353 single-nucleotide polymorphisms were identified in total. The table displays a selection of rare non-conservative coding variants in targets of antimicrobial chemotherapy and antibiotic resistance determinants.