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. 2016 Aug 31;6(1):63. doi: 10.1186/s13568-016-0233-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Protein and zymogram analysis of Spr Cel8A in 10 % SDS-PAGE. a Analysis of total protein extracts of transformed E. coli M15 [pREP4] /pQE30/spr cel8A, M: MW protein standards; line 1 and 2 non-induced soluble fraction medium and bacterial cell harvested respectively; line 3 induced soluble fraction medium; line 4 and 5 induced lysed bacterial cells soluble fraction. b Purification of recombinant Spr Cel8A; line 1 total protein extracts of transformed E. coli M15 [pREP4] /pQE30/Spr Cel8A and line 2 Spr Cel8A purified. c Zimogram analysis of Spr Cel8A in 10 % SDS-PAGE with 2 % CMC