Fig. 2.

Presence of Al³⁺ in nuclei isolated from C. arabica protoplasts treated with AlCl₃. Before treatment with 100 µM aluminum (0, 5, 10, 20 or 30 min), the protoplasts were exposed to 50 μM morin for 1 h. After exposure to morin and AlCl₃, protoplasts were exposed to DAPI for 20 min. The nuclei were obtained as outlined in the “Methods” section. Morin and DAPI staining were observed at 470–520 and 365 nm, respectively, using a fluorescence microscope. Bars 40 μm