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. 2016 Feb 29;18(2):90–99. doi: 10.1016/j.neo.2015.12.003

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Treatment of FTECs with FF induces AID expression. (A) AID transcription induction following 4 h incubation with individual FF and pooled FF. (B) Time course of AID induction following FF pool stimulation from 15 min to 24 h (15 min n = 3, Wilcoxon test, P = .64, 45 min n = 5, P = .007, 2 h n = 4, P = .28, 4 h n = 7, P = .003, 24 h n = 3, P = .06). (C) Increased AID protein expression in the cytoplasm of FTECs following stimulation with FF pool for 4 h as observed by immunohistochemistry (× 20 magnification (top) and × 40 magnification (bottom)). Increased AID protein expression following incubation with FF pool as observed by western blot analysis in FTECs (D), and in iFTECs (E).