Skip to main content
. 2016 Feb 29;18(2):111–120. doi: 10.1016/j.neo.2016.01.002

Figure 1.

Figure 1

Ezrin and EGFR interacts in NSCLC cell lines. (A) H292 cells were serum starved overnight and pretreated with 5 μM ezrin inhibitor NSC305787 or DMSO for 2 hours followed by 100-ng/ml EGF treatment for 5 minutes. Cell lysates were immunoprecipitated using an antiezrin antibody or control mouse total IgG. Precipitates were evaluated by Western blotting using anti-EGFR (upper panel) and antiezrin (lower panel) antibodies. EGFR interaction with ezrin was enhanced with EGF stimulation, and NSC305787 inhibited this interaction in both the presence and absence of the ligand. (B) Immunofluorescence staining of EGFR (red), ezrin (green), and DAPI (blue) in DMSO-treated (upper images) and NSC305787-treated (bottom images) A549 cells was analyzed by fluorescence microscopy. Ezrin and EGFR colocalization (yellow) in the membrane (arrow) and perinuclear area (arrow head) is indicated. NSC305787 treatment inhibited the colocalization of ezrin and EGFR.