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. 2016 Feb 29;18(2):111–120. doi: 10.1016/j.neo.2016.01.002

Figure 3.

Figure 3

Inhibition of ezrin expression causes decreased levels of EGFR in nucleus in NSCLC cells. (A) Ezrin expression was inhibited using siRNA oligos in A549 and H292 cells, and whole cell lysates were subjected to cell fractionation and immunoblotting with antiezrin and anti-EGFR antibodies. Lamin A/C and tubulin antibodies were used as control for nuclear and non-nuclear fractions, respectively. (B) Immunofluorescence staining of EGFR, ezrin, and DAPI in control siRNA–treated (left panel) and ezrin siRNA–treated (right panel) A549 cells. Nuclear and perinuclear EGFR localization was decreased in ezrin-depleted cells. (C) Cells were serum starved for 24 hours and treated with 5 μM NSC305787 or DMSO for 2 hours before EGF stimulation for 30 minutes. NSC305787 decreased EGF-induced nuclear translocation of EGFR in H292 cells.