Fig. 9.

HIF-1α activation supports extracellular acidosis. A: immunoblot analysis of HIF-1α (arrow) in the nuclear and cytoplasmic fractions from vector control (Hygro) or PDGF-B BPH-1 cells with vehicle only or 100 μM CoCl₂ treatment. Histone H1 and PTEN were used as loading controls for the nuclear and cytoplasmic fractions, respectively. Upon treatment of vector (Hygro) or PDGF-B BPH-1 cells with CoCl₂, CA mRNA expression (RT-PCR) (B), changes in extracellular pH (C–F), and matriptase activation (G) were monitored. Values represent the mean ± SD. *P < 0.05. Ponceau S was used to evaluate equal gel loading.