Fig. 8.

CaSR detection by proximity ligation assay in mouse, rat, and human kidney. Photomicrographs are shown of CaSR immunolocalization by proximity ligation assay in mouse (A), rat (C), and human (E) cortical kidney sections. Immunoperoxidase staining (brown dots) indicates the proximity between the epitopes detected by the C-term1/N-term6 antibody pair in mouse and rat sections and by the full length/N-term6 antibody pair in human sections. Photomicrographs are also shown of the negative controls performed by incubation with isotype control or omission of a primary antibody (mouse, B; rat, D; human, F). Arrow indicates TAL, and asterisk indicates PT. Scale bar = 50 μm.