Fig. 9.

CaSR expression in rat kidney CD. Photomicrographs are shown of CaSR mRNA expression in the cortical CD detected by in situ hybridization (ISH; A). Positive signal (as detected by ISH) corresponds to fast red staining. Photomicrographs of CaSR protein expression in the cortical CD detected by IHC with N-term1 (Thermo; B), N-term2 (Anaspec; C), C-term1 (W. Chang; D) or proximity ligation assay with C-term1/N-term6 (Alomone; E) are also shown. F: photomicrographs of CaSR protein expression in outer medullary CD detected by PLA with C-term1/N-term6. Positive signal corresponds to immunoperoxidase staining (brown), and nuclei are counterstained with hematoxylin (blue) in IHC and PLA images. Scale bar = 50 μm.