Table 1.
Details of antibodies used in the study
| Epitope |
|||||||
|---|---|---|---|---|---|---|---|
| Antibody | Manufacturer | Reactivity | Species | Region | Host | Clone | IHC Dilution |
| N-term1 | Abcam (ab19347)/Thermo (MA1-934)* | Mouse, rat, human | Human | N-term (ADD) N'-ADDDYGRPGIEKFREEAEERDI-C' | Mouse | Mono | 1:500 |
| N-term2 | Anaspec† (53286) | Mouse, rat, human | Rat | N-term | Rabbit | Poly | 1:500 |
| N-term3 | W. Chang (custom made) | Mouse, rat, human | Human | N-term (ADD) N'-DYGRPGIEKFREEAEE-C' | Mouse | Mono | Not tested |
| N-term4 | W. Chang (custom made) | Mouse, rat, human | Human | N-term (ADD) N'-DYGRPGIEKFREEAEE-C' | Mouse | Mono | Not tested |
| N-term5 | W. Chang (custom made) | Mouse, rat, human | Human | N-term (ADD) N'-ADDDYGRPGIEKFREEAEERDI-C' | Rabbit | Poly | Not tested |
| N-term6 | Alomone (ACR-004) | Mouse, rat, human | Human | N-term (ADD) | Rabbit | Poly | 1:500 |
| C-term1 | W. Chang (custom made) | Mouse, rat | Mouse | C-term N'-NSEDRFPQPERQKQ-C' | Mouse | Mono | 1:150 |
| C-term2 | LS Bio‡ (LS-C117834) | Mouse, rat, human | Human | C-term aa854-903 | Rabbit | Poly | Not tested |
| Full length | Novus Bio (H00000846-B01P) | Human | Human | Full length | Mouse | Poly | 1:75 |
*
N-term1 is a monoclonal antibody produced using the 5C10, ADD clone and commercialized by different companies, including Abcam, Thermo-Scientific, Novus Bio, Genetex (Irvine, CA), and Acris (Herford, Germany).
†
Anaspec, Fremont, CA.
‡
LS Bio, Seattle, WA.