Fig. 4.

Deregulation of the neural precursor cell expressed developmentally downregulated protein (NEDD)4-2 (N4-2)/with no lysine kinase (WNK)1 pathway in Sgk1^Pax8/LC1 mice after 2 days of the HKD. A: WB analysis of NEDD4-2 and WNK1 revealing a significant decrease in total NEDD4-2 and pNEDD4-2 levels in Sgk1^Pax8/LC1 mice. A slight (nonsignificant) alteration was observed in WNK1 total protein levels. B: protein quantification of the results shown in A (n = 7 and 6 mice/group). *P < 0.05; **P < 0.01. C: IF images of NEDD4-2 in control and Sgk1^Pax8/LC1 mutant mice showing a decrease of total NEDD4-2 expression in KO mice. D: costaining of the Na⁺-Cl⁻ cotransporter (NCC; red; top) and WNK1 (green; middle). The punctate pattern of WNK1 observed in the distal convoluted tubule (DCT) of control animals disappeared in Sgk1^Pax8/LC1 animals, where WNK1 exhibited a diffused pattern. E: costaining of aquaporin (AQP)2 (red) and WNK1 (green) showing the decrease in WNK1 expression in the connecting tubule (CNT)/collecting duct (CD) of mutant compared with control animals.