Table 1.
Constructs used in this study.
| Plasmid | Description | Source |
|---|---|---|
| pJRS525 | E. coli / S. pyogenes shuttle vector | McIver and Scott, 1997 |
| pSL230 | pJRS525 with scl1.41 allele | Caswell et al., 2007 |
| pSL501 | pJRS525 with scl1.3WT allele | Flores et al., 2015 |
| pSL502 | pSL501 with repaired allele scl1.3FL | Flores et al., 2015 |
| pSL518 | pSL502 with KanR in place of SpcR | This study |
| pASK-IBA2 | E. coli expression vector | IBA, Göttingen |
| pSL154 | pASK-IBA2 encoding rScl1.3V | This study |
| pSL503 | pASK-IBA2 encoding rScl1.3FL | Flores et al., 2015 |
| pSL514 | pASK-IBA2 with rScl2.3 | This study |
| pSB027 | GFP-encoding plasmid | Cramer et al., 2003 |