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. 2016 Aug 31;7:281. doi: 10.3389/fphar.2016.00281

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Copyright © 2016 Tighilet, Dutheil, Siponen and Noreña.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Study designs. The first protocol (#1) was elaborated using BrdU and Ki67 as cell proliferation markers to study the time-dependent effect of a UCN on reactive cell proliferation in cochlear nuclei. Protocol number 2 (#2) was designed using a continuous infusion of NaCl, AraC, or Muscimol in the fourth ventricle to examine alterations in newborn cell survival (BrdU marker), astrogenesis (GFAP marker), GABA cells (GAD67 marker), neuronal cells (NeuN marker), and microglial cells (IBA1 marker) in the cochlear nuclei. BrdU, 5-bromo-2′deoxyuridine; i.p., intraperitoneal injection; GAD67, glutamic acid decarboxylase; GFAP, glial fibrillary acidic protein; CN, cochlear nuclei; n = 4 animals per group.