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. 2016 Aug 31;7:281. doi: 10.3389/fphar.2016.00281

FIGURE 2.

FIGURE 2

Reactive cell proliferation occurs after unilateral cochlear neurectomy (UCN) in a time-sensitive manner. (A) Photomicrographs showing 5-bromo-2′-deoxyuridine (BrdU) immunostainings in the deafferented cat AVCN and PVCN submitted to UCN and analyzed at post-lesion days 1, 3, and 30 (D1, D3, and D30, respectively). Animals received a BrdU injection (i.p., 200 mg/kg body weight) 3 h before sacrifice. Scale bar: 100 μm for lower magnification. (B) Quantitative evaluation. Histograms comparing the mean values (±Standard Error of the Mean, SEM) of the number of BrdU-immunopositive cells in the deafferented CN at D1, D3, and D30 after UCN. Different letters indicate significant differences between groups of animals and assessed by ANOVA followed by the Scheffé test, p < 0.0001, see Table 1. a: significantly different from control, UCN d3, UCN d7, UCN d15, and UCN d30 groups. b: significantly different from control, UCN d1, UCN d7, UCN d15, and UCN d30 groups. Only values recorded on the lesioned side are illustrated. n = 4 animals per group.