Table 1.

High-throughput sequencing platforms used for Lymphocyte Repertoire Study.

HTS platform	Mechanism	Read depth	Read length	Typical throughput	Covered region	Application	Accuracy
Roche 454 GS FLX Titanium XLR70	Pyro-sequencing	~1 million	Up to 600 bp	450 Mb	FR1-C region	BCR (long read), TCR (long read)	Consensus accuracy 99.995%
Illumina HiSeq	Dye terminator sequencing	~2 billion	2 × 250 bp	500–1000 Gb	FR3-C region	TCR (CDR3), BCR (long read), TCR (long read)	>80% bases above Q30
Illumina MiSeq	Dye terminator sequencing	~25 million	2 × 150 bp	13.2–15 Gb	FR1-C region	TCR (CDR3)	>80% bases higher than Q30 at 2 × 150 bp
			2 × 300 bp				>75% bases higher than Q30 at 2 × 250 bp
Ion Torrent/Life Technologies	Semiconductor sequencing	~1 billion	Up to 200 bp	30 Mb–2 Gb	FR3-C region	TCR	>97% bases