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. 2016 Aug 31;7:1387. doi: 10.3389/fmicb.2016.01387

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Copyright © 2016 Wang, Liu, Xu, Yang, Wang, Han, Shi, Tian, Ding, Peng and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transmission electron micrographs of APEC strains. Bacteria were grown overnight on LB agar, and then harvested, fixed, and negatively stained with 2% phosphotungstic acid. Numerous long flagella were observed on the surfaces of the wild-type strain APCE94 (A) and the complementation strain APCE94CΔeivC (C). Few flagella were observed on the mutant strain APCE94ΔeivC (B). Augmented fimbriae surrounding the bacteria were observed on the mutant strain APCE94ΔeivC (E), compared with those of the wild-type strain APCE94 (D) and the complementation strain APCE94CΔeivC (F).