Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Characteristics | Reference |
|---|---|---|
| Strains | ||
| APCE94 | APEC wild-type strain, O78 serotype | This study |
| APCE94ΔeivC | eivC deletion mutant in APCE94 | This study |
| APCE94CΔeivC | APCE94ΔeivC with plasmid pSTV28-eivC | This study |
| DH5α | F-, Δ(lacZYA-argF)U169, recA1, endA1, hsdR17 (rk-, mk+), phoA, supE44, λ- | TIANGEN |
| BL21 (DE3) | F-, ompT, hsdS (rB- mB-) gal, dcm (DE3) | TIANGEN |
| Plasmids | ||
| pET28a(+) | Kan, F1 origin, His tag | Novagen |
| pET28a-eivC | pET28a (+) carrying eivC gene | This study |
| pSTV28 | Cm, lacZ | Takara |
| pSTV28-eivC | pSTV28 derivative harboring eivC gene | This study |
| pKD46 | Amp; expresses λ red recombinase | Datsenko and Wanner, 2000 |
| pKD3 | Cm gene, template plasmid | Datsenko and Wanner, 2000 |
| pCP20 | Cm, Amp, yeast Flp recombinase gene, FLP | Datsenko and Wanner, 2000 |