Figure 4.

Increased expression of genes involved in zinc and iron uptake and mobilization in M. tuberculosis lag phase. Shown are changes of gene expression in the regulons of Zur and IdeR in regrowth cultures at time points between D1 and D0, D2 and D0, or D2 and D1. Changes were identified by enrichment analysis using the transcription regulatory network. Data were derived from the RNA-Seq data of the reactivating bacilli from three independent cultures at each time point. Green color indicates up-regulation and red color denotes down-regulation. To show the connections between the two regulons, also included are genes (marked with asterisk) whose expression showed more than 1.5-fold change but did not reach significant level (p ≤ 0.05) in corresponding time points.