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Instrument and working conditions for ginsenoside analysis by high-performance liquid chromatography (HPLC)
| Instrument | Shimadzu LC-10AT HPLC system | ||
|---|---|---|---|
| Column | YMC AM303, 4.6 × 250 mm | ||
| Detector | UV/VIS detector (203 nm) | ||
| Solvent A | H2O | ||
| Solvent B | Acetonitrile | ||
| Injection volume | 20 μL | ||
| Flow rate | 1 mL/min | ||
| Gradient elution system | %A | %B | |
| Time (min) | 0 | 20 | 80 |
| 30 | 20 | 80 | |
| 35 | 35 | 65 | |
| 60 | 65 | 45 | |
| 65 | 20 | 80 | |
| 70 | 20 | 80 | |
UV/VIS, Ultraviolet/visible.
