Figure 3.

Gene expression of MORN5 in later stages of chicken embryo. (A–C) ISH analysis in frontal sections of chicken head at stage HH20. There was strong expression in the maxillary prominence. The expression appeared in the cranial part in each mandibular prominence and it continued in dorsal direction. (B) No expression was observed in mesenchymal condensations and close to the fusion region of mandibular prominences. (E–G) MORN5 expression in frontal section of chicken head at stage HH24. MORN5 expression was strong in the mesenchyme of maxillary prominences. MORN5 expression was localized in the dorsal part of mandibular prominence, but not in mesenchymal condensation and close to the midline. (I–K) Frontal sections of chicken head at stage HH27 showed prominent expression in the maxillary prominence. There was weak expression in the globular process. Prominent expression was observed in rostral part of maxillary prominence and also in the mandibular prominence with the exception of midline. (M–O) Frontal sections of chicken head at stage 29 where beak is evident. MORN5 expression was localized in rostral part of maxilla and in fusion region. In the mandibular prominence, there was strong expression but not in the midline. (D,H,L,P) ISH analysis using sense probe. FNM, frontonasal mass; GP, globular process; mc, mesenchymal condensation; MdP, mandibular prominence; MxP, maxillary prominence. Scale bars = 100 μm.