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. 2016 Aug 31;7:351. doi: 10.3389/fphys.2016.00351

Figure 5.

Figure 5

Podocin is required for 20-HETE modulation of podocyte TRPC6 channels. (A) Representative immunoblots showing podocin abundance in cells treated with non-targeting siRNA and siRNA targeting podocin. Bar graph summarizing three repetitions of this experiment is shown to the right. Asterisk indicates P < 0.01 (unpaired t-test). (B) Treatment with siRNA targeting podocin does not affect TRPC6 abundance. Representative immunoblots are shown to the left, and bar graph summarizing results from three different transfections is shown to the right. (C) Representative trace showing increased currents in podocyte evoked by 1 μM 20-HETE in cell treated with non-targeting siRNA. (D) Typical response to 1 μM 20-HETE in cell treated with siRNA targeting podocin. (E) Summary of fold increase in current at +80 mV evoked by 1 μM 20-HETE compared to baseline in cells treated with non-targeting siRNA (control) and siRNA targeting podocin. Asterisk indicates P < 0.05 (unpaired t-test, n = 6 cells per group).