Table 1.
Summary of sequencing and de novo assembly.
| Sequences | 105d | 115d | 125d | 135d |
|---|---|---|---|---|
| BEFORE TRIMMING | ||||
| Total nucleotides (bp) | 8,394,207,768 | 8,690,289,268 | 8,280,370,668 | 7,478,728,618 |
| Number of raw reads | 41,555,484 | 43,021,234 | 40,991,934 | 37,023,409 |
| AFTER TRIMMING | ||||
| Number of clean reads | 41,067,827 | 42,872,666 | 40,886,898 | 36,927,707 |
| GC content (%) | 46.96 | 47.07 | 47.03 | 46.54 |
| Q30 percentage (%) | 86.11 | 86.02 | 86.46 | 87.49 |
| AFTER ASSEMBLY | ||||
| Number of transcripts of combined data | 100,711 | |||
| Number of unigenes of combined data | 52,093 | |||
| Total nucleotides (nt) of transcripts (bp) | 132,974,754 | |||
| Total nucleotides (nt) of unigenes (bp) | 45,459,242 | |||
| Mean length of transcripts (bp) | 1320 | |||
| Mean length of unigenes (bp) | 872 | |||
| N50 of unigenes (bp) | 1675 | |||