Table 1.

Summary of immunization regimen of macaques used in this study.

Immunization group	Weeks 0 and 4 (IM)	Week 12 (IT)	Weeks 24 and 36 (IM)	Week 48 (IR)
1. DNA (n = 7)	DNA-SIVa plasmids + control DNA	Ad5hr-SIVb recombinants	SIV proteinsc	SIVmac251d
2. DNA/IL-12 (n = 7)	DNA-SIV plasmids + DNA/IL-12	Ad5hr-SIV recombinants	SIV proteins	SIVmac251
3. DNA/IL-15 (n = 7)	DNA–SIV plasmids + DNA/IL-15	Ad5hr-SIV recombinants	SIV proteins	SIVmac251

^aThree DNA-SIV plasmids separately encoding SIV_mac239env, SIV_mac239gag, and SIV_mac239rev/nef were administered together by the indicated route at 2.5 mg of DNA per dose. IM, intramuscular.

^bThree Ad5hr-SIV recombinants separately encoding SIV_smH4 env/rev; SIV_mac239 gag; and SIV_mac239 nef_Δ1–13 were administered at 5 × 10⁸ pfu/recombinant/route. IT, intratracheal.

^cSIV_mac251 gp140; and SIV_mac239 Nef proteins were given at a dose of 100 and 50 μg, respectively, in MPL-SE adjuvant.

^dChallenge administered at 10 50% monkey infectious doses. IR, intrarectal.