Table 1.
Protocol | Target | Primers or probe | PCR | Amplicon size (bp) | Reference |
---|---|---|---|---|---|
kDNA | Kinetoplast DNA | 121 122 |
C | 330 – T. cruzi 330 to 380 – T. rangeli |
Schijman et al., 2003; Burgos et al., 2007; Botero et al. 2010 |
SatDNA | Satellite DNA | cruzi1 cruzi2 cruzi3 |
RT | 166 – T. cruzi |
Piron et al., 2007; Schijman et al., 2011; Duffy et al., 2013 |
RibDNA | Ribosomal DNA | D75 D76 |
C | 270 – T. cruzi I 290 – T. cruzi II-V-VI 240 – T. rangeli |
Souto et al., 1999; Schijman et al., 2006; Burgos et al., 2007 |
C: Conventional PCR
RT: Real Time PCR