Table 3.
Gene predictors of heart mass are not members of a single biological process
| Illumina | Gene | Coefficient | Illumina | Gene | Coefficient |
|---|---|---|---|---|---|
| ILMN_2934549 | BC055324 | −6.79E-05 | ILMN_2444594 | Cdh3 | −2.17E-05 |
| ILMN_2816271 | Dcpp2 | 6.22E-05 | ILMN_2890915 | EG331493 | −2.15E-05 |
| ILMN_2687716 | 4930433I11Rik | −5.99E-05 | ILMN_2751822 | Dsg1b | 2.11E-05 |
| ILMN_3072147 | Foxo6 | −5.77E-05 | ILMN_2509644 | Tpk1 | 2.10E-05 |
| ILMN_2482494 | Trim16 | 5.75E-05 | ILMN_2795644 | Tlr11 | −2.06E-05 |
| ILMN_2977849 | Fank1 | −4.57E-05 | ILMN_1253791 | Kcnv2 | −2.05E-05 |
| ILMN_1234692 | 1300007F04Rik | 4.29E-05 | ILMN_2719803 | Stap1 | 1.82E-05 |
| ILMN_2780323 | Tkt | 4.27E-05 | ILMN_2859908 | Olfr335 | −1.79E-05 |
| ILMN_2792089 | Srebf1 | −4.21E-05 | ILMN_2686924 | Epha1 | 1.78E-05 |
| ILMN_1213351 | Gsdma1 | 4.10E-05 | ILMN_3162671 | EG432555 | −1.75E-05 |
| ILMN_1238397 | Olfr126 | −4.01E-05 | ILMN_2824954 | OTTMUSG00000000421 | −1.65E-05 |
| ILMN_2742887 | Scrib | 3.89E-05 | ILMN_2540726 | Olfr670 | −1.60E-05 |
| ILMN_3072427 | Il1rn | 3.70E-05 | ILMN_2703720 | Bclaf1 | 1.55E-05 |
| ILMN_2965612 | Abca6 | −3.55E-05 | ILMN_2701712 | Plcxd3 | −1.48E-05 |
| ILMN_1223335 | Ano10 | −3.52E-05 | ILMN_1254646 | Sox6 | −1.48E-05 |
| ILMN_2734661 | Hagh | 3.49E-05 | ILMN_2806235 | Gm813 | 1.44E-05 |
| ILMN_1240675 | Rbm12b | 3.43E-05 | ILMN_2911123 | Itga2b | −1.40E-05 |
| ILMN_1214602 | Sfrp2 | 3.12E-05 | ILMN_3062163 | Rab11fip5 | 1.38E-05 |
| ILMN_2642418 | Mest | −3.10E-05 | ILMN_2675623 | Mrfap1 | −1.32E-05 |
| ILMN_1245040 | 9030617O03Rik | −3.02E-05 | ILMN_1218034 | Tmco4 | 1.31E-05 |
| ILMN_2898062 | Olfr108 | 2.96E-05 | ILMN_1239583 | Wins2 | −1.29E-05 |
| ILMN_1213265 | 2610208M17Rik | 2.96E-05 | ILMN_2863437 | 1110038F14Rik | −1.19E-05 |
| ILMN_2833993 | 4921501E09Rik | −2.91E-05 | ILMN_3048689 | Rffl | −1.19E-05 |
| ILMN_2779272 | Olfr313 | −2.86E-05 | ILMN_2805051 | Upk3a | 1.18E-05 |
| ILMN_2708142 | Xkr6 | 2.82E-05 | ILMN_2693946 | Olfr1347 | −9.91E-06 |
| ILMN_2753867 | Scgb3a2 | 2.76E-05 | ILMN_2742311 | Cyp39a1 | −9.27E-06 |
| ILMN_2670398 | Eif4ebp1 | 2.70E-05 | ILMN_1214065 | Slco1a6 | −8.32E-06 |
| ILMN_2900653 | Gadd45b | 2.69E-05 | ILMN_1223591 | Zfp202 | −7.79E-06 |
| ILMN_2938373 | Tas2r116 | −2.68E-05 | ILMN_2601758 | Gsto2 | −6.97E-06 |
| ILMN_2682493 | Bmp5 | 2.57E-05 | ILMN_2678714 | Id4 | 6.61E-06 |
| ILMN_1259759 | Olfr672 | 2.57E-05 | ILMN_2657207 | Hey2 | −5.61E-06 |
| ILMN_2527490 | LOC381375 | 2.51E-05 | ILMN_2661495 | Tmem44 | 3.59E-06 |
| ILMN_1242281 | Txndc2 | 2.43E-05 | ILMN_2726837 | Nppb | 3.50E-06 |
| ILMN_1221960 | Gtf2ird1 | −2.38E-05 | ILMN_2700468 | Pcdhgc4 | −3.37E-06 |
| ILMN_2790241 | Herpud1 | −2.28E-05 | ILMN_2705242 | Wee2 | 2.22E-06 |
| ILMN_2630521 | Hist1h1a | −2.24E-05 | ILMN_2863849 | C1qtnf3 | 2.19E-06 |
| ILMN_1223384 | Nadsyn1 | 2.20E-05 | ILMN_2596998 | Lypd6b | 1.67E-06 |
Linear regression analysis, using the glmnet package, was performed on change in expression with isoproterenol for all probes on the microarray to predict change in normalized total heart mass in 82 strains. We used 10-fold cross-validation to build each model, and a total of 1,000 models were performed to identify 98 probes with predictive capacity in 800 of the 1,000 models. The model incorporates 74 of these probes and fits the actual data for the 82 strains with an r-squared of 0.99. Coefficient indicates weight of the contribution in final model. GO analysis on these genes returned no significant enrichment for biological processes or cellular components.