Figure 5.

Intracellular Localization of SEC61A1 in Affected Kidney

(A and D) In affected kidney, SEC61A1 is present in coarsely granular structures.

(B, C, E, and F) Co-staining of SEC61A1 with Protein disulphide isomerase (PDI) (B), a marker of endoplasmic reticulum (ER), and with 58k Golgi-protein (E) demonstrate localization of the SEC61A1 in the ER (C) and in the Golgi (F).

(G and J) In control kidney, SEC6A1A is present in finely granular structures.

(H, I, K, and L) Co-staining with (H) PDI and (K) GM130 demonstrating localization of SEC61A1 in ER (I) but not in the Golgi (L). The degree of SEC6A1A colocalization with selected markers is demonstrated by the fluorescent signal overlap coefficient values that ranging from 0 to 1. The resulting overlap coefficient values are presented as the pseudo color which scale is shown in corresponding lookup table.