TABLE 3.
Comparison of NTHi culture and fucP/hpd#3 qPCR quantification in clinical specimens
| Semiquantitative NTHi culture score (no.)a | fucP/hpd#3 qPCR geometric mean quantity of DNA (pg/μl) (95% CIb) |
|---|---|
| +++ (8) | 6.58 (−4.13 to 30.13) |
| ++ (18) | 1.45 (0.15 to 8.87) |
| + (30) | 0.18 (0.17 to 2.35)c |
| 0 (23) | 0.01 (−0.07 to 0.24)d |
a
One culture-positive specimen was excluded from analysis as a semiquantitative culture was not recorded.
b
95% CI, 95% confidence interval.
c
Includes 2 H. influenzae culture-positive specimens that were less than the LOQ for both targets. Specimens in which both targets were not detected were assigned half of the LOQ for statistical analyses.
d
Two of the culture-negative specimens were qPCR positive.