Table 1.
Overview of bacterial species, corresponding genes, primers/probes, and DNA sequences
| Species | Gene | Primer/probe | Sequences (5′-3′) |
|---|---|---|---|
| Mycoplasma salivarium | rpoB | msali-F | CCG TCA AAT GAT TTC GAT TGC |
| msali-R | GAA CTG CTT GAC GTT GCA TGT T | ||
| msali-S | Hex-ATG ATG CTA ACC GTG CGC TTA TGG GTG-BHQ1 | ||
| Veillonella parvula | 16S rDNA | vpar-F | TGC TAA TAC CGC ATA CGA TCT AAC C |
| vpar-R | GCT TAT AAA TAG AGG CCA CCT TTC A | ||
| vpar-S | HEX-CTA TCC TCG ATG CCG A-BHQ1 | ||
| Staphylococcus aureus | nuc | saur-F | CAA AGC ATC CTA AAA AAG GTG TAG AGA |
| saur-R | TTC AAT TTT CTT TGC ATT TTC TAC CA | ||
| saur-S | FAM-TTT TCG TAA ATG CAC TTG CTT CAG GAC CA-BHQ1 | ||
| Porphyromonas gingivalis | Arg-gingipain | pgin-F | CCT ACG TGT ACG GAC AGA GCT ATA |
| pgin-R | AGG ATC GCT CAG CGT AGC ATT | ||
| pgin-S | TexRed-TCG CCC GGG AAG AAC TTG TCT TCA-BHQ2 | ||
| Parvimonas micra | 16S rDNA | pmic-F | TCG AAC GTG ATT TTT GTG GAA A |
| pmic-R | GGT AGG TTG CTC ACG TGT TAC TCA | ||
| pmic-S | FAM-CCC GTT CGC CAC TT-BHQ1 | ||
| Tannerella forsythia | bspA | tfor-F | TCC CAA AGA CGC GGA TAT CA |
| tfor-R | ACG GTC GCG ATG TCA TTG T | ||
| tfor-S | FAM-CCG CGA CGT GAA ATG GTA TTC CTC-BHQ1 | ||
| tfor-S II | HEX-TCG CGA CGT GAA ATG GTA TTC CTC-BHQ1 |