Table 2.

Primers used in qPCR

Gene	Sense primers (5′→3′)	Anti-sense primers (5′→3′)	Fragment length (bp)	Annealing temperature (°C)	Mean Ct BT	Mean Ct AT
IL-1β	ATGATGGCTTATTACAGTGGCAA	GTCGGAGATTCGTAGCTGGA	132	57	38.9	37.7
IL-10	GACTTTAAGGGTTACCTGGGTTG	TCACATGCGCCTTGATGTCG	112	58	34.1	36.6
TNF-α	GAGGCCAAGCCCTGGTATG	CGGGCCGATTGATCTCAGC	91	57	37.6	37.7
IFN-γ	TCGGTAACTGACTTGAATGTCCA	TCGCTTCCCTGTTTTAGCTGC	93	57	31.9	34
IRS-2	CCCGACTTCTTCTCCGCAG	GAAGGCACTACAGGGTGAGG	124	58	35.1	35.3
MMP-9	TGGGCTACGTGACCTATGACAT	GCCCAGCCCACCTCCACTCCTC	128	54	32.2	34.9
β-actin	GCTTCTTTGCAGCTCCTTCGT	ATATCGTCATCCATGGCGAAC	150	56	32.6	35.6

Ct, threshold cycle; BT, before training; AT, after training.