Figure 6.

NL1 deletion impairs NMDAR-mediated EPSCs elicited by short stimulus trains in stellate cells. A, Sample traces of EPSCs in response to five APs at 100 Hz recorded without (trace i) or with (trace ii) CNQX at a +60 mV holding potential in cerebellar stellate cells from P21–P23 littermate control (black traces) and PV-Cre NL1 mice (red traces). The current without CNQX (trace i) is mixture of AMPAR- and NMDAR-mediated currents, whereas the current in the presence of CNQX (trace ii) is mediated exclusively by NMDARs. AMPAR-mediated currents were calculated by subtracting trace ii from trace i. Arrows indicate the current at 100 ms after the last EPSC peak. B, Summary graphs of NMDAR- and calculated AMPAR-mediated EPSCs. Data are means ± SEM. Numbers in bars represent the number of cells/mice used. Statistical significance was determined by Student's t test.