Figure 7.

Neuroligin deletion significantly decreases NMDAR-mediated currents induced by puffing glutamate onto cerebellar stellate cells, but has no effect on AMPAR-mediated currents. A, Illustration of the recording configuration. B–D, Sample traces and summary graph of NMDAR-mediated EPSCs induced by local puffing of glutamate (50 mm, injected at 8 psi for 5 ms) onto cerebellar stellate cells from littermate control (black traces) and PV-Cre NL123 mice (red traces) at P21–P23. CNQX (20 μm), picrotoxin (50 μm), and TTX (1 μm) were added both to the extracellular and the puffing solutions to block AMPAR-mediated and inhibitory currents. E–G, Same as B–D, but for AMPAR-mediated EPSCs induced by local puffing of glutamate. D-AP5 (50 μm), picrotoxin (50 μm), and TTX (1 μm) were added both to the extracellular and the puffing solutions to block NMDAR-mediated and inhibitory currents. Data are means ± SEM. Numbers in bars represent the number of cells/mice used. Statistical significance was determined by Student's t test or one-way ANOVA.