Figure 5.

Nodes of Ranvier are reassembled in regenerating CNS axons. A–F, Representative images of optic nerves immunostained for βIV spectrin (green, arrows) and Caspr (red) proximal to the crush (A, C, E) and at the crush site (B, D, F). Optic nerves were examined at 2, 6, and 12 weeks after crush. G, H, The ratio of nodes of Ranvier per FOV in crushed optic nerve to uninjured control optic nerve at 2, 6, and 12 weeks after crush. Measurements were made proximal to the crush site (−325 μm) and within the crush site (0 μm). Treatment = Cre+zymosan+cAMP. Scale bars: A–F, 10 μm. *p < 0.05 (unpaired t test with Mann–Whitney post test).