Fig. 3.
a A multinucleated TRAP-positive osteoclast was observed by TRAP staining. Cells containing tartrate-sensitive acid phosphatase are devoid of activity, and only the cells containing tartrate acid-resistant phosphatase show maroon dye deposits at the sites of activity. Staining confirmed that the presence of TRAP-positive multinucleated osteoclast-like cells was limited to CTR− PBMCOVX, CTR+ PBMCOVX, and PBMCSHAM. b RGB evaluation of TRAP staining after 1, 2, and 3 weeks of culture (mean ± SD, n = 4 triplicates). CTR−: basal culture medium (without RANKL, M-CSF, PTH); CTR+: differentiated medium (with RANKL, M-CSF, PTH). Scheffé post hoc multiple comparison test between PBMCOVX and PBMCSHAM for each culture condition: 1st week, CTR− PBMCOVX versus PBMCSHAM ***p < 0.0005; 2nd week, CTR− PBMCOVX versus PBMCSHAM ***p < 0.0005; 3rd week, CTR− PBMCOVX versus PBMCSHAM ***p < 0.0005