Table 4.
The relative protein levels of InsR, IGF-1R, BDNF, Syt-1, and Stx-1 indicated by AOD of immunohistochemical staining (×100)
| Proteins | Groups | DG | CA1 | CA3 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HL | GL | ML | ML | RL | PL | OL | ML | SL | PL | OL | ||
| InsR | O-con | 2.17 ± 0.33 | 4.66 ± 0.83 | 2.06 ± 0.42 | 3.15 ± 0.61 | 1.72 ± 0.44 | 4.60 ± 0.76 | 2.65 ± 0.60 | 1.90 ± 0.48 | 3.24 ± 0.71 | 6.20 ± 0.51 | 3.33 ± 0.42 |
| Y-con | 4.88 ± 0.43** | 8.62 ± 0.76** | 4.12 ± 0.51** | 6.07 ± 0.66* | 4.72 ± 0.53** | 8.18 ± 0.75** | 5.90 ± 0.67** | 4.77 ± 0.57** | 5.43 ± 0.51** | 8.56 ± 0.36** | 6.28 ± 0.45** | |
| HD-DNJ | 3.26 ± 0.37 | 6.41 ± 0.51 | 2.92 ± 0.39 | 5.90 ± 0.47* | 2.99 ± 0.50 | 7.05 ± 0.57 * | 4.25 ± 0.58* | 3.34 ± 0.43* | 3.69 ± 0.44 | 6.29 ± 0.65 | 4.79 ± 0.38* | |
| LD-DNJ | 2.86 ± 0.49 | 5.44 ± 0.93 | 2.19 ± 0.32 | 3.85 ± 0.51 | 2.11 ± 0.38 | 6.03 ± 0.59 | 3.44 ± 0.56 | 2.56 ± 0.51 | 2.76 ± 0.66 | 5.70 ± 0.65 | 3.02 ± 0.43 | |
| IGF-1R | O-con | 2.65 ± 0.32 | 3.00 ± 0.34 | 2.65 ± 0.32 | 3.11 ± 0.59 | 2.75 ± 0.51 | 4.29 ± 0.74 | 3.99 ± 0.84 | 1.81 ± 0.45 | 2.61 ± 0.46 | 4.52 ± 0.71 | 2.31 ± 0.44 |
| Y-con | 0.99 ± 0.41* | 1.59 ± 0.74 | 1.06 ± 0.39* | 1.31 ± 0.33* | 0.47 ± 0.19** | 2.67 ± 0.57 | 0.84 ± 0.31** | 0.61 ± 0.11* | 1.22 ± 0.19* | 3.87 ± 0.66 | 1.09 ± 0.22* | |
| HD-DNJ | 1.48 ± 0.41* | 2.09 ± 0.45 | 2.04 ± 0.45 | 2.41 ± 0.46 | 1.32 ± 0.40* | 3.71 ± 0.61 | 2.46 ± 0.47 | 1.12 ± 0.30 | 1.76 ± 0.41 | 3.28 ± 0.61 | 1.46 ± 0.26 | |
| LD-DNJ | 1.82 ± 0.62 | 2.29 ± 0.68 | 1.86 ± 0.50 | 2.26 ± 0.52 | 1.75 ± 0.46 | 4.26 ± 0.59 | 2.51 ± 0.54 | 1.31 ± 0.55 | 1.81 ± 0.39 | 3.93 ± 0.59 | 1.28 ± 0.38* | |
| BDNF | O-con | 3.04 ± 0.34 | 2.26 ± 0.40 | 2.55 ± 0.36 | 3.88 ± 0.54 | 3.16 ± 0.52 | 3.24 ± 0.41 | 2.23 ± 0.34 | 2.52 ± 0.45 | 3.64 ± 0.44 | 4.77 ± 0.56 | 3.35 ± 0.48 |
| Y-con | 4.22 ± 0.64 | 5.25 ± 0.69** | 4.40 ± 0.64* | 5.37 ± 0.56 | 5.05 ± 0.66 | 5.65 ± 0.52** | 3.78 ± 0.57 | 3.72 ± 0.60 | 5.29 ± 0.54* | 7.62 ± 0.49** | 4.93 ± 0.59 | |
| HD-DNJ | 3.70 ± 0.77 | 5.83 ± 1.04** | 4.59 ± 0.84* | 5.28 ± 0.78 | 4.81 ± 0.78 | 6.24 ± 0.70** | 3.82 ± 0.62 | 3.69 ± 0.69 | 5.34 ± 0.84 | 6.87 ± 0.66* | 5.20 ± 1.22 | |
| LD-DNJ | 3.64 ± 0.71 | 5.06 ± 1.05* | 3.96 ± 0.68 | 4.15 ± 0.79 | 3.40 ± 0.54 | 5.08 ± 0.78* | 3.07 ± 0.71 | 2.80 ± 0.69 | 3.47 ± 0.70 | 5.24 ± 0.76 | 3.20 ± 0.77 | |
| Syt-1 | O-con | 7.03 ± 1.48 | 1.32 ± 032 | 3.53 ± 0.82 | 3.16 ± 0.81 | 6.65 ± 1.22 | 2.15 ± 0.41 | 6.30 ± 1.17 | 4.24 ± 0.76 | 4.92 ± 0.54 | 2.58 ± 0.51 | 5.30 ± 0.83 |
| Y-con | 4.11 ± 1.15 | 0.16 ± 0.08** | 0.95 ± 0.15** | 0.53 ± 0.05** | 1.07 ± 0.31** | 0.27 ± 0.07** | 1.06 ± 0.23** | 0.89 ± 0.20** | 1.08 ± 0.26** | 0.31 ± 0.10** | 1.29 ± 0.22** | |
| HD-DNJ | 4.53 ± 0.92 | 0.17 ± 0.06* | 0.96 ± 0.18* | 0.58 ± 0.26* | 2.49 ± 0.67* | 0.50 ± 0.16* | 2.57 ± 0.95* | 1.05 ± 0.43* | 1.75 ± 0.51* | 0.68 ± 0.13* | 1.94 ± 0.61* | |
| LD-DNJ | 6.92 ± 1.01 | 0.49 ± 0.10* | 2.10 ± 0.18* | 1.64 ± 0.36* | 4.63 ± 0.77 | 0.78 ± 0.16* | 5.03 ± 0.80 | 1.88 ± 0.40* | 3.85 ± 0.89 | 1.27 ± 0.34* | 3.70 ± 0.48 | |
| Stx-1 | O-con | 0.88 ± 0.06 | 0.15 ± 0.05 | 0.42 ± 0.12 | 0.34 ± 0.11 | 0.45 ± 0.11 | 0.03 ± 0.00 | 0.25 ± 0.08 | 0.24 ± 0.08 | 0.63 ± 0.08 | 0.03 ± 0.00 | 0.47 ± 0.15 |
| Y-con | 1.76 ± 0.14* | 0.33 ± 0.12 | 0.57 ± 0.19 | 0.68 ± 0.18 | 1.40 ± 0.22** | 0.13 ± 0.05* | 0.67 ± 0.19* | 0.77 ± 0.21* | 1.17 ± 0.21 | 0.05 ± 0.01 | 0.75 ± 0.20 | |
| HD-DNJ | 1.41 ± 0.19* | 0.11 ± 0.05 | 0. 56 ± 0.18 | 0.56 ± 0.22 | 1.22 ± 0.14* | 0.06 ± 0.02 | 0.34 ± 0.13 | 0.56 ± 0.10* | 1.14 ± 0.22 | 0.03 ± 0.01 | 0.72 ± 0.18 | |
| LD-DNJ | 1.32 ± 0.16* | 0.07 ± 0.01 | 0.32 ± 0.09 | 0.37 ± 0.08 | 0.66 ± 0.09 | 0.04 ± 0.01 | 0.22 ± 0.06 | 0.28 ± 0.08 | 0.75 ± 0.14 | 0.02 ± 0.01 | 0.52 ± 0.04 | |
| GFAP | O-con | 2.72 ± 0.60 | 1.47 ± 0.39 | 2.30 ± 0.43 | 3.54 ± 0.74 | 2.01 ± 0.46 | 1.44 ± 0.48 | 1.95 ± 0.28 | 1.92 ± 0.59 | 1.37 ± 0.35 | 0.35 ± 0.08 | 2.09 ± 0.60 |
| Y-con | 1.12 ± 0.39* | 0.48 ± 0.15* | 0.59 ± 0.22** | 1.46 ± 0.55* | 0.92 ± 0.20* | 0.25 ± 0.06* | 1.31 ± 0.32 | 0.78 ± 0.20* | 0.50 ± 0.14* | 0.25 ± 0.08 | 0.69 ± 0.11* | |
| HD-DNJ | 1.31 ± 0.21 | 0.76 ± 0.22 | 0.86 ± 0.15* | 0.93 ± 0.20** | 0.95 ± 0.12* | 0.29 ± 0.06* | 0.99 ± 0.12* | 0.70 ± 0.17* | 0.62 ± 0.14 | 0.39 ± 0.10 | 0.54 ± 0.09* | |
| LD-DNJ | 1.14 ± 0.15* | 1.15 ± 0.15 | 1.02 ± 0.26* | 0.90 ± 0.13** | 1.13 ± 0.15 | 0.49 ± 0.10 | 1.22 ± 0.25 | 0.79 ± 0.12* | 0.71 ± 0.17 | 0.41 ± 0.08 | 1.06 ± 0.23 | |
| H4K8ac | O-con | – | 2.04 ± 0.08 | – | – | – | 1.76 ± 0.09 | – | – | – | 1.34 ± 0.09 | – |
| Y-con | – | 3.85 ± 0.09** | – | – | – | 2.86 ± 0.10** | – | – | – | 2.84 ± 0.10** | – | |
| HD-DNJ | – | 3.19 ± 0.09** | – | – | – | 2.65 ± 0.08** | – | – | – | 2.58 ± 0.11** | – | |
| LD-DNJ | – | 2.10 ± 0.10 | – | – | – | 2.01 ± 0.08 | – | – | – | 1.62 ± 0.08* | – | |
Data are expressed as mean ± SEM
DG dentate gyrus, HL Hilus, GL granule cell layer, ML molecular layer, RL radiation layer, PL pyramidal cell layer, OL original layer, SL stratum lucidum, O-con old controls, Y-con young controls
*P < 0.05, **P < 0.01 denote significant difference compared to the old controls