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. 2016 Jan 29;38(1):17. doi: 10.1007/s11357-016-9878-1

Fig. 2.

Fig. 2

Autofluorescent, label-free images of the lamina propria (LP) (imaged from the urothelial side) in the bladder wall of a 25-week-old, b 52-week-old, and c 85-week-old mice. a Collagen, below the urothelium, could be seen in the second, spectral channel (Co). Vessel structures with a diameter ranging from 10 to 40 μm were found (white asterisk). b Collagen could be detected with a strong second harmonic generation signal (Co, white asterisk). Also, blood vessel could be seen in the LP (white arrow). c An increase of amount and density of collagen was noticeable, reaching from the sub-urothelium, throughout the LP to the smooth muscle layer (Co). The connective tissue appeared multi-layered, with a layer existing of rather straight-shaped collagen (Co, white asterisk) bundles (20–40 μm), followed by a thick layer of curled collagen (Co, red asterisk) bundles (40–80 μm). Vessel structures in the LP were more dilated and were showing a diameter of up to approx. 70 μm (white arrow). Macrophages were also found (red arrow) and confirmed with MOMA-1 immuno-histochemical staining. Scale bars, 20 μm