Fig. 4.

Evaluation of healthspan in C. elegans exposed to UV-A. a Pharyngeal pumping assay in C. elegans. The pharyngeal movement was reduced in worms exposed to UV-A (p < 0.05). b Brood size assay in C. elegans. The total number of eggs laid was 227, 166, and 118 in 2, 4, and 6 h exposed worms, respectively, when compared to control (314) (p < 0.05). c qPCR expression of egl-8 and egl-30 was found to be downregulated from the initial hours of exposure which showed slight upregulation during the course of exposure. In the case of dgk-1 and goa-1, it was upregulated during the initial hours which was later subsided