Fig. 5.

SASP effect on L929 proliferation. L929 mice fibroblasts were exposed up to 72 h to SASP-MEM media (1:1) from primary fibroblast. Proliferation was determined as described in “Materials and methods” section. Control cells were cultured with the conditioned media from non-senescent cells (C-NS). Each bar represents the mean ± SE from three independent determinations in triplicate. *Statistical significance against untreated cells at the same day (p < 0.05)