Figure 2.

Removing unintended events from an FC sample. (A) Single intact cells (inside the red polygon gate) are separated from the doublets (outside of the red polygon gate). (B) A viability marker (ViViD) is used to remove dead cells (outside of the red polygon gate). (C) Cells emitting very low or very high fluorescence signals (outside of the red vertical lines) are removed as potential outlying events.