Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 31;6:188. doi: 10.3389/fonc.2016.00188

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Azad, Rajwa and Pothen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) A predefined rectangular gate (red rectangle on the lower left corner) denotes an approximate boundary for the lymphocytes. (B) Inside the rectangular gate, lymphocytes are identified as a dense and normally distributed region (red ellipse). (C) Outlying cells fall outside of a pair of predefined thresholds shown with the red vertical lines and are removed. (D) Correlated CD4 and CD8 expressions due to the spectral overlap between PE and ECD fluorochromes. (E) CD8 vs. CD4 expressions after spectral unmixing. The inverse hyperbolic sine (asinh) transformation is used in (D,E) for visualization.