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. 2016 Aug 31;6:188. doi: 10.3389/fonc.2016.00188

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Copyright © 2016 Azad, Rajwa and Pothen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stabilizing the within-cluster variance for each channel of the HD dataset. (A) Variances of the clusters increase monotonously with their means before the variances are stabilized. Clusters in each marker are shown with the same symbol and color. (B) Variances are approximately stabilized for each marker/channel after the data are transformed by the asinh function with the optimum cofactor. (C) Density of the variance-stabilized fluorescence channels are plotted where different subjects are denoted with different colors.