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(A) Simultaneous optimization of five cluster validation criteria suggests that four cell populations are present in this sample. Here, three of the indices are maximized and two are minimized. (B) Bivariate projections of cell populations display four subsets of lymphocytes: red (natural killer cells), blue (B cells), black (helper T cells), and green (cytotoxic T cells). Each cell cluster is CD45+ since we pre-selected lymphocytes on the forward and side scatter channels.
