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. 2016 Aug 31;7:117. doi: 10.3389/fendo.2016.00117

Figure 1.

Figure 1

Spine density analysis of enhanced green fluorescent protein (EGFP)-expressing gonadotropin-releasing hormone (GnRH) neurons from P0 males of maternally vehicle (VEH-P0)- and dexamethasone-treated (DEX-P0). Projected confocal images and binary processing for three-dimensional rendering of EGFP–GnRH neurons of (A,B) VEH-P0 and (C,D) DEX-P0 males showing spines (white arrow) and filopodia (white arrowhead) on the GnRH neuronal soma and along the primary dendrite. The number of spines emerging from the soma and primary dendrite of GnRH neurons in the MS (E), OVLT (G), and AHA (I) did not differ between VEH-P0 and DEX-P0 males (n = 6/group). Small increase in the number of filopodia on the GnRH neuronal soma was found in the MS (F) of DEX-P0 males. However, the number of filopodia emerging from the soma and primary dendrite of GnRH neurons in the OVLT (H) and AHA (J) region did not differ between VEH-P0 and DEX-P0 males. MS, medial septum; OVLT, organum vasculosum of the lamina terminalis; AHA, anterior hypothalamic area. Data are represented by the mean ± SEM for each group. ***P < 0.05 compared to VEH-P0 group. Scale bar = 10 μm (A,C), 5 μm, and 20 μm (B,D).