Fig. 2.

Effects of burn trauma on mammalian target of rapamycin (mTOR) signaling in skeletal muscle. Phosphorylation of mTOR at Ser²⁴⁴⁸ (A), eukaryotic initiation factor 4E binding protein (4E-BP1) at Thr^37/46 (B), S6rp at Ser^240/244 (C), and eukaryotic elongation factor 2 (eEF2) at Thr⁵⁶ (D) was significantly greater in burn patients, whereas phosphorylation of p70S6K at Thr³⁸⁹ (E), eukaryotic elongation factor factor-2 kinase at Ser³⁶⁶ (Eef2k; F), and eEF2 at Ser⁵¹ (G) did not differ between groups. Data were normalized to a loading control (GAPDH; n = 4 healthy controls and n = 5 burn patients). Values are presented means ± SE. P < 0.05 vs. control.