Table 2.
Primers used for RT-PCR of total RNA
| Gene (Amplicon) | DNA Sequence |
|---|---|
| Human IGF-I (173 bp) | |
| Exon 1 | 5′-TTGCTAAATCTCACTGTCACTGC |
| Exon 4 | 5′-GCTCCGGAAGCAGCACT |
| Human IGF-I (122 bp) | |
| Exon 2 | 5′-TGGATGCTCTTCAGTTCGTG |
| Exon 4 | 5′-GCTCCGGAAGCAGCACT |
| Human IGF-I (119 bp) | |
| Exon 3 | 5′-GATGCTCTTCAGTTCGTGTGT |
| Exon 4 | 5′-GCTCCGGAAGCAGCACT |
| Mouse Igf1 (213 bp) | |
| Exon 3 | 5′-CTCTTCAGTTCGTGTGTGGACC |
| Exon 4 | 5′-GTCTTGGGCATGTCAGTGTGG |
| Mouse Igfals (246 bp) | |
| Exon 2 | 5′-GATAGCATCCCAGTCAGCA |
| Exon 3 | 5′-TGAGTGAAGCCAGACTTGGT |
| Mouse Socs2 (212 bp) | |
| Exon 3 | 5′-GAATGGAGCGGACAGGAC |
| Exon 4 | 5′-ATCCTGTTTGACTGAG |
| Mouse Mrps17 (328 bp) | |
| Exon 2 | 5′-ATCCCCAGCAAGAAGCTTCGGAACA |
| Exon 3 | 5′-TATGGCATAACAGATTAAACAGCTC |
Socs2, suppressor of cytokine signaling 2; Mrps17, mitochondrial ribosomal protein S17; Igfals, insulin-like growth factor-binding protein acid labile subunit.